蜂胶总黄酮对四氧嘧啶糖尿病大鼠降血糖作用

杨明,隋殿军*,陈文学,杨铭,于德伟

中国药学杂志 ›› 2014, Vol. 49 ›› Issue (16) : 1410-1413.

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中国药学杂志 ›› 2014, Vol. 49 ›› Issue (16) : 1410-1413. DOI: 10.11669/cpj.2014.16.011
论著

蜂胶总黄酮对四氧嘧啶糖尿病大鼠降血糖作用

  • 杨明1,隋殿军2*,陈文学1,杨铭1,于德伟1
作者信息 +

Hypoglycemic Effect of the Total Flavones of Propolis on Diabetic Model Induced by Alloxan in Rats

  • YANG Ming1, SUI Dian-jun2*, CHEN Wen-xue1, YANG Ming1, YU De-wei1
Author information +
文章历史 +

摘要

目的 探讨蜂胶总黄酮(TFP)对四氧嘧啶(ALX)糖尿病模型大鼠降血糖作用及其机制。方法 采用四氧嘧啶(150 mg·kg-1)腹腔注射法建立糖尿病大鼠模型,根据血糖值随机分为模型对照组、蜂胶总黄酮高剂量组(240 mg·kg-1)、蜂胶总黄酮中剂量组(120 mg·kg-1)和蜂胶总黄酮低剂量组(60 mg·kg-1),并将同批未经上述处理的大鼠作为正常对照组,每组10只大鼠。期间蜂胶总黄酮高、中、低剂量组每天灌胃给药1次,正常对照组与模型对照组给予同体积0.5%羧甲基纤维素钠(CMC-Na),连续4周。每周测血糖1次,末次给药结束后,禁食12 h,乙醚麻醉,取肝组织测肝糖原,腹主动脉取血,检测糖尿病大鼠血清中血糖(GLU)、胰岛素(INS)、C肽(C-P)、糖化血红蛋白(GHb)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)及肝糖原水平。结果 与正常对照组大鼠比较,模型对照组大鼠血糖、糖化血红蛋白、一氧化氮及丙二醛水平明显升高(P<0.05或P<0.001),胰岛素、C肽、超氧化物歧化酶、谷胱甘肽及肝糖原含量明显降低(P<0.01或P<0.001)。与模型对照组比较,蜂胶总黄酮高、中、低剂量组血糖、糖化血红蛋白、一氧化氮及丙二醛水平明显降低(P<0.05或P<0.01或P<0.001),胰岛素、超氧化物歧化酶、谷胱甘肽及肝糖原含量明显升高(P<0.05或P<0.001),而C肽仅在蜂胶总黄酮高、中剂量组明显升高。结论 蜂胶总黄酮可通过增加机体的抗氧化能力,促进胰岛细胞分泌功能恢复而发挥降血糖作用。

Abstract

OBJECTIVE To investigate the hypoglycemic and mechanism effect of the total flavones of propolis(TFP) in diabetic model rats induced by alloxan. METHODS Diabetic model was reproduced by intraperitoneal injection of alloxan(ALX) (150 mg穔g-1). Then the diabetic rats were randomly divided into four groups by blood glucose, the diabetic model control group, high (240 mg穔g-1) dose of the TFP group, medium (120 mg穔g-1) dose of the TFP group, low (60 mg穔g-1) dose of the TFP group, in control group the rats were not treated by ALX, continuously treated for 4 weeks, and both the control and model groups were given equal 0.5% sodium carboxymethylcellulose(CMC-Na) by intragastric administration. The levels of glucose(Glu), insulin(INS), serum C-peptide(C-P),glycated hemoglobin(GHb), nitric oxide(NO), superoxide dismutase(SOD), malondialdehyde(MDA), glutathione(GSH), hepatic glycogen were measured at the experiment. RESULTS Compared with the normal group, the levels of GLU, GHb,NO and MDA were increased obviously (P<0.05 or P<0.001), and the levels of INS,C-P,SOD, GSH and hepatic glycogen were significantly decreased (P<0.01 or P<0.001) in groups of model group. Compared with the model group, the levels of GLU, GHb,NO and MDA were decreased significantly (P<0.05 or P<0.01 or P<0.001), and the levels of INS,C-P,SOD, GSH and hepatic glycogen were increased significantly on the high-dose group, medium-dose group and low-dose group (P<0.05 or P<0.001). The level of C-P was increased significantly on the high-dose group, medium-dose group. CONCLUSION TFP might enhance islet cell secreting function to recovery to reduce the level of GLU by antioxidant effect.

关键词

蜂胶总黄酮 / 四氧嘧啶 / 降血糖 / 氧化应激

Key words

total flavones of propolis / alloxan / hypoglycemic / oxidative stress

引用本文

导出引用
杨明,隋殿军*,陈文学,杨铭,于德伟. 蜂胶总黄酮对四氧嘧啶糖尿病大鼠降血糖作用[J]. 中国药学杂志, 2014, 49(16): 1410-1413 https://doi.org/10.11669/cpj.2014.16.011
YANG Ming, SUI Dian-jun*, CHEN Wen-xue, YANG Ming, YU De-wei. Hypoglycemic Effect of the Total Flavones of Propolis on Diabetic Model Induced by Alloxan in Rats[J]. Chinese Pharmaceutical Journal, 2014, 49(16): 1410-1413 https://doi.org/10.11669/cpj.2014.16.011
中图分类号: R965   

参考文献

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基金

国家“十一五”重大新药创制(2009ZX09103-418);吉林省科技支撑计划(20110946)
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